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KMID : 0604220060130010021
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Korean Journal Investigative Dermatology
2006 Volume.13 No. 1 p.21 ~ p.30
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Analysis of Genes with Changed Expression in UVB-iradiated Human Skin HaCaT Keratinocytes and Fibroblast
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Song Hyun-Jeong
Cho Jae-We
Lee Kyu-Suk
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Abstract
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Background: Ultraviolet B(UVB) irradiation is an important inducer of several biologic changes in skin, including sunburn, premature skin aging, and skin cancer, which are mainly mediated by direct DNA damage or production of reactive oxygen species. These processes are closely related with changes of expressions of many genes in cells. However it is largely unknown the expression profiles of genes in UVB- irradiated keratinocytes and fibroblasts.
Objective: To investigate the expression profiles of genes in UVB-irradiated keratinocytes and fibroblasts, thereby to generally evaluate biological response to UVB in genetic level.
Methods: We cultured the human skin HaCaT keratinocytes and fibroblasts. The cells were irradiated by UVB(100mJ/cm2), and then the expression profiles of genes in cells were evaluated using a cDNA microarray representing 25,000 genes.
Results: The number of genes up-regulated and down-regulated over 2 folds is 691 in HaCaT keratinocytes, 597 in fibroblasts and 174 in both. In HaCaT keratinocytes, the expression of apoptosis regulator, cancer and transport associated genes is dominant. Otherwise, the expression of structural protein associated genes is dominant in fibroblasts.
Conclusion: These results provide the basis for understanding the effect of UVB on human skin keratinocytes and fibroblasts in genetic level.
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KEYWORD
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Ultraviolet B, Keratinocytes, Fibroblasts, cDNA microarray
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